中国环境科学

作者：习彦花, 王馨芝, 李旭, 魏帅强, 孙立博, 吕亚天, 程辉彩

单位：1. 河北省科学院生物研究所, 河北 石家庄 050081;2. 河北工程大学园林与生态工程学院, 河北 邯郸 056038;3. 河北地质大学水资源与环境学院, 河北 石家庄 050031

关键词：纳米四氧化三铁,牛粪,高浓度,厌氧发酵,抗生素抗性基因

基金：河北省重点研发项目(22373803D);河北省科学院科技计划项目(22302,23302,24302)

In order to improve the biogas production performance of high-concentration anaerobic fermentation of cow manure, the enhancement effect of different concentrations of nano iron trioxide (Nano-Fe3O4) on substrate hydrolysis and biogas production efficiency was tested, and the influence on microbial community structure and antibiotic resistance genes (ARGs) in the fermentation system were further explored. The results showed that adding 500mg/L of Nano-Fe3O4 can improve the hydrolysis degree of high concentration anaerobic fermentation substrates in cow manure, enhance system stability, and increase biogas production by 12.36%. Compared with the control, the abundance of Firmicutes and Fibrobacters in the fermentation system significantly increased by 37.39% and 75.86%, respectively. At the genus level, especially the abundance of Clostridium and Ruminococcus was 3.50 times and 8.69 times higher than that before. Methanosarcina was dominant among methanogens,with an abundance of 2.55%, a significant increase of 27.50% over the control. In addition, the abundance of potential pathogenic bacteria Treponema and Acinetobacter was significantly decreased by 72.14% and 93.69%, respectively. The reduction rate of tetracyclines (tetA,tetG,tetH,tetJ) and aminoglycosides (aph(3), aph(6)) in ARGs was 100%; The average reduction rate of sulfonamide class (sul1, sul2) was 74.41%~96.55%. Research has shown that adding an appropriate amount of Nano-Fe3O4 had multiple promoting effects on stabilizing the fermentation system, improving microbial activity, increasing biogas production, and reducing antibiotic resistance genes, etc. The research results can provide relevant theoretical guidance and practical basis for the use of conductive exogenous regulatory additives such as Nano-Fe3O4 in livestock and poultry manure biogas projects.

来源：2024年第11期

《中国环境科学》期刊编辑部

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