中国环境科学

作者：刘文信, 温群伟, 周旭, 吴清文, 徐阳, 秦春燕, 陈雪岚, 黄明珠

单位：1. 江西师范大学, 国家淡水鱼加工技术研发专业中心, 江西 南昌 330022;2. 江西师范大学生命科学学院, 江西 南昌 330022;3. 江西师范大学健康学院, 江西 南昌 330022

关键词：铜耐受,铜吸附,转录组,能量代谢,金属离子转运

基金：江西省自然科学基金资助面上项目(20232BAB205003);江西省自然科学基金资助青年项目(20224BAB215004);江西省自然科学基金资助重点项目(20202ACBL205001);国家自然科学基金资助项目(32260014)

We isolated a copper-resistant strain from a copper-contaminated area. The 18S and 26S rRNA sequencing showed it had the closest genetic relationship with Cryptococcus laurentii. It was found that the strain could grow normally in the medium with 15mmol/L Cu2+, and the maximum adsorption of copper was 29.36μmol/L/OD. In addition, the transcriptome analysis of the strains after copper stress showed that 126genes were up-regulated and 143genes were down-regulated; multiple genes related to amino acid metabolism, energy metabolism, and metal ion transport pathways were differentially expressed. At the same time, we found that copper stress blocked the glucose metabolism pathway, the expression of metabolism related genes decreased by 37% to 73%, which may be one of the important reasons why copper inhibited the growth of bacteria. The expression of heavy metal transport-related functional genes increased dramatically by 7 to 26 times in response to copper stress when induced by copper. The purpose of this study is to reveal the transcriptional response of the strain to copper stress and the metabolic pathways that may be involved in copper resistance, and to provide a basis for the application of microbial technology in copper pollution remediation.

来源：2024年第11期

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